Journal: Frontiers in Immunology
Article Title: Decidual stromal cells drive CD16 + macrophages towards an immunoregulatory phenotype via extracellular matrix-adhesion molecule interaction during early pregnancy
doi: 10.3389/fimmu.2025.1747323
Figure Lengend Snippet: Patients RM exhibit weakened interactions between DSCs and CD16 + dMφ. (A) Compared the expression of COL4A1 in decidua from women with normal pregnancy (NP, n=8) or recurrent miscarriage (RM, n=8) by Immunohistochemistry. Scale bar, 50 μm. (B) Compared the expression of OPN in decidua from women with NP (n=10) or RM (n=10) by Immunohistochemistry. Scale bar, 50 μm. (C) Compared the expression of HA in decidua from women with NP (n=8) or RM (n=8) by Alcian blue staining assays. Scale bar, 50 μm. (D) Levels of CD16, CD86, CD206 and CD209 in CD45 + CD14 + dMφ from women with NP (n=7) or RM (n=7) by flow cytometry assays. The data are presented as the mean ± SEM; two-tailed Student’s t -test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Article Snippet: DMφ from DICs were obtained through positive selection by an CD14 + cell isolation kit according to the reagent instructions (Miltenyi Biotec, 130–050-201).
Techniques: Expressing, Immunohistochemistry, Staining, Flow Cytometry, Two Tailed Test